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Nuclear Medicine Updated 2026-04

Bone Scan — Tc-99m MDP Scintigraphy Interpretation

Bone scan interpretation: 3-phase protocol, osteomyelitis vs cellulitis distinction, superscan, flare phenomenon, cold lesions, and pattern-based differential diagnosis.

Quick summary

Tc-99m MDP (methylene diphosphonate) accumulates at sites of osteoblastic activity proportional to bone turnover and blood flow. The 3-phase protocol separates vascular, inflammatory, and metabolic causes of increased uptake: Flow (2–3 sec/frame × 60 frames) → Blood pool (5–10 min post-injection) → Delayed whole body (3–4h). SPECT or SPECT/CT adds anatomic localization.

Normal: Symmetric skeletal uptake, kidneys and bladder visible, mild growth plate activity in children.

Patterns and Interpretation

Pattern Differential / Interpretation Key Pearls
Focal hot spot (delayed) Metastasis, fracture, osteomyelitis, Paget's disease, arthritis — non-specific Most common pattern; CT/MRI correlation required for specificity
3-phase positive (↑ flow + ↑ blood pool + ↑ delayed at same focal site) Osteomyelitis — all 3 phases elevated at infection site Sens ~90%, Spec ~73%; compare with cellulitis: flow + blood pool elevated but delayed normal/mildly ↑
Flow + blood pool ↑, delayed normal or mildly ↑ Cellulitis — no osseous involvement Key distinction from osteomyelitis; if delayed becomes focally hot, consider underlying bone infection
Cold / photopenic defect Early AVN, aggressive lytic tumor, multiple myeloma, radiation field Early AVN: cold on bone scan — MRI gold standard; myeloma: cold or normal (unlike most metastases)
Superscan — diffusely increased skeletal uptake; absent or faint renal/soft tissue activity Diffuse bone metastases (prostate, breast), metabolic bone disease (renal osteodystrophy, hyperparathyroidism) Can appear deceptively "normal" — absent kidney activity is the key finding; correlate with PSA/tumor markers
Flare phenomenon Transient worsening of bone scan 2–6 months after starting effective chemotherapy — represents healing, NOT progression Correlate with tumor markers and CT; serial scans show improvement after 6 months

Superscan pitfall: Diffuse metastatic disease can look deceptively "normal" — the skeleton is uniformly hot and the kidneys are absent or faint. Always assess renal activity. If kidneys are not visible on a whole-body bone scan, consider superscan.

Osteomyelitis vs. cellulitis: The key is the delayed phase. Both elevate flow and blood pool. Osteomyelitis elevates all three phases at the same focal site. Cellulitis shows diffuse soft-tissue phase uptake that does NOT focally concentrate on delayed images. When in doubt, MRI provides definitive soft tissue and marrow characterization.


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